Optimization and Validation of an HPLC Method for Assessing the Purity Profile of Chosen Antiviral and Anticancer Medication

Abstract

High-Performance Liquid Chromatography (HPLC) plays a critical role in pharmaceutical analysis by ensuring the accurate assessment of drug purity profiles. This abstract reviews the optimization and validation of HPLC methods specifically tailored for selected antiviral and anticancer medications.The methodological optimization involves meticulous selection of chromatographic conditions, including stationary phase, mobile phase composition, and detection wavelength, to achieve optimal separation and quantification of drug components. Validation parameters such as accuracy, precision, linearity, specificity, and robustness are rigorously evaluated to ensure the reliability and reproducibility of results, meeting regulatory standards.comparative analyses highlight the diverse approaches and challenges encountered in HPLC methodology for antiviral and anticancer drugs. Recent technological advancements in HPLC, including enhanced column chemistry and sensitive detectors, contribute to improved resolution and detection limits, thereby supporting more efficient drug development and quality control processes.this review consolidates current knowledge and practices in HPLC methodology for assessing the purity profile of antiviral and anticancer medications, emphasizing its pivotal role in pharmaceutical research, development, and regulatory compliance.